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Image Search Results
Journal: Pharmaceutics
Article Title: Surface-Available HER2 Levels Alone Are Not Indicative of Cell Response to HER2-Targeted Antibody–Drug Conjugate Therapies
doi: 10.3390/pharmaceutics16060752
Figure Lengend Snippet: HER2 copy numbers on cell surfaces determined by flow cytometry. Violin plots demonstrate the distribution of HER2 expression among cell lines; this distribution is especially prevalent in the HER2-positive cell lines (BT-474, SK-BR-3, NCI-N87, and SK-OV-3). For each cell line, N = 9. Cells were fixed prior to staining with T-AF647 in excess. The passage numbers were 6–12. All data points from flow cytometry were analyzed, and outliers were identified with a Q = 1 test. Violin plots show the median (dashed line) and 95% CI (dotted lines). ns = not significant, ** p < 0.01, **** p < 0.0001, Tukey.
Article Snippet: AF647 fluorescence intensity was calibrated using the
Techniques: Flow Cytometry, Expressing, Staining